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Cleanert DNPH-Silica

Summary

Cleanert DNPH-Silica prepared by acidified dinitrophenylhydrazine reagent coated on silica is used for collection of air samples.

Cleanert DNPH-Silica prepared by acidified dinitrophenylhydrazine reagent coated on silica is used for collection of air samples. It was one of most sensitive and specific method for analyzing aldehydes and ketones which is based on their reaction with 2,4-dinitrophenylhydrazine (DNPH) and subsequent analysis of the hydrazone derivatives by HPLC. DNPH-Silica can be used in EPA Method TO?1A; ASTM D5197 for carbonyl compounds in air and JPMOE Official Methods for aldehydes: odor in out door air and in exhaust gas.

Specification
▲ Ave Background value: ≤0.1 μg (calculated by formaldehyde)
▲ Max sampling quantity: ≥75 μg (calculated by formaldehyde)
▲ Max Sampling Pressure: 0.15 MPa
▲ Recoveries: >90%

3 mL DNPH cartridge

Left: 200 mg/1 mL
Right: 350 mg/1 mL

Operation
Using a vacuum pump, the air sample is drawn through the new Cleanert® DNPH-silica cartridge. The aldehydes and ketones react with the DNPH and form the hydrazone derivative, which is retained on the cartridge. After sampling, the hydrazones are eluted from the cartridge with acetonitrile and analyzed by HPLC.

Ozone has been shown to interfere with the analysis of carbonyl compounds in air samples. Cleanert® Ozone remover cartridge contains potassium iodide could be used connected with Cleanert® DNPH to remove ozone interferences.


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