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Venusil ASB Series Columns

Product Detail

The Venusil ASB series columns are specially designed for the separation of polar compounds from low (extremely stable at pH=1.0) to medium pH.

The Venusil® ASB series columns are specially designed for the separation of polar compounds under low (extremely stable at pH=0.8)to medium pH condition. The stationary phase is bonded with unique bulky silanes that sterically protect the siloxane bond. We offer a line of bonding chemistry of C1, C8, C18 or Phenyl groups presenting a broad selection of different polarity for various applications.

Stationary Phase Characteristics

Bonded phase

Particle size(um)

Pore Size(Å)

Carbon loading(%)

Specific surface(m2/g)

End-capping

pH range*

C18

3,5,10

150

12

200

No

0.8-7.5

C8

3,5

150

7

200

No

1.0-7.5

C1

5

150

3

200

No

1.0-7.5

phenyl

5

150

6

200

No

1.0-7.5

C18(T)

5

150

5

80

No

0.8-7.5

Notes: * Optimum pH range of ASB series is 1.0-4.0; Use the column under optimum pH could ensure a longer lifetime.

Main Features

● Low pH stability, keep stable even at pH=0.8

● Peak shape and efficiency: Excellent peak symmetry for basic compounds comparing to other non end-capped phase columns

● Excellent separation ability for polar compounds

● 100% aqueous compatible: Much better peak shape, retention and efficiency for polar compounds

● Five different bonded phases provide broad selectivity

The Stability in the Low pH

Column: Venusil® ASB C18, 4.6×150 mm, 5 μm

Sample: Naphthol

Aging: 40°C, TFA in 80% methanol (pH=1.0), 400 hours

Mobile Phase: TFA in 80% methanol (pH=1.5)

Flow Rate: 1 mL/min

Injection: 5 μL

Temperature: 30°C

Column: Venusil® ASB C18, 4.6×150 mm, 5 μm

Sample: Vc, malonic acid, lactic acid and citric acid

Mobile Phase: 20 mM Phosphate buffer saline (PBS) ,

pH=2.0

Flow Rate: 1 mL/min

Injection: 5 μL

Detector: UV 210 nm

Highest LC-MS Sensitivity

2-3 times higher (low bleeding, inert surface and high efficiency)

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