Bonna-Agela's unique sol-gel process technology generates an uniform surface that produces an universal column
applicable to several kinds of HPLC applications.
Peak tailing adversely affects chromatographic efficiency and reduces the accuracy and precision of the data. Peak
tailing and insufficient retention of polar compounds are among the toughest problems for silica based reversed phase columns. High acidity of the surface silanols, non-uniformity of the surface, and non-uniformity of the bonding process are the three major causes of peak tailing.
Synthetic silica with high purity is typically obtained by hydrolyzing silicic acid esters followed by high temperature
sinteration. The sintered silica is further leached with dilute acid to enrich silanol on the surface. The process does not generate a uniform, silanol covered surface. It will create some isolated silanol groups with very strong acidity or some "un-leached islands" during the leaching process. Furthermore, the high temperature procedure may cause the formation of micro-crystallization domains on the silica, further contributing to the nonuniformity of the surface.
Unisol process technology is specifically designed to solve these problems. In our patented Unisol process, gas phase tetramethoxy silane is adsorbed evenly on the silica surface. It is subsequently hydrolyzed by a controlled amount of moisture. A fresh layer of silanols is then formed by a mild heating process. This process produces a surface with low acidity and high uniformity, which is further bonded with a C18 silane, and then di-functional end-capped. The overall process yields an excellent HPLC stationary phase with a very smooth surface that is friendly to basic compounds (minimal ionic interactions) and polar compounds (provides significantly increased retention).
● Greatly improved peak shape for basic compounds
● 100% water compatibility
● Significantly increased retention for highly water soluble compounds
and balanced hydrophobicity in the reverse phase mode
● Elimination of excessive retention for polar compounds in the normal
phase mode (few 'hot spots')
Unisol C18 (2) Cloumns
Unisol C18 (2) column is an universal reversed phase column through carefully
optimizing the pore size, surface and carbon content and the Bonna-Agela’s
innovative surface modification and unique bonding processes. It has excellent
balance retention for hydrophilic and hydrophobic analytes and also balance of
separation ability and contamination tolerance. In addition, the Unisol C18 (2)
column demonstrates good retention capability for compounds with different
polarity. Because the ionized silanols on the stationary surface of Unisol
C18(2) are eliminated maximumly, so this column is less affected by the pH of
mobile phase and buffer concentration. Finally, it is able to provide excellent
Unisol C18(2): Metal Impurity<30 ppm; Pore Size: 110 Å; Specific Surface Area: 340 m2/g;
Available Particle Size: 3 μm, 5 μm or 10 μm; Double end-capped; Carbon Loading: 14%;
pH range is 1.5 to 9.0, and optimized pH is 2.0-6.0, which could ensure a satisfied lifetime.
● High density of deionized silanol: 100 % water compatible and enhanced retention and separation for polar isomer
● Reduce of ionized silanols and blockage of metal ions: good peak shape for basic compounds
● Reduce of the quantity of ion exchange sites: less affected by the pH mobile phase and buffer concentration.
● Weakened nonspecific adsorption: Good tolerance of biological matrix.
The activity testing of silicon hydroxyl
The Unisol C18 (2) columns provide symmetric peak and good resolution for acidic, neutral and basic compounds at
usual pH range. Retention of basic compound is less changed when the pH of mobile phase changes
Sample: Uracil, Phenol, dimethylphthalate, naphthalene, amitriptyline
Mobile Phase: Methanol:20mM potassium phosphate buffer (pH2.6/pH5.8) =70: 30
Flow Rate: 1.00 mL/min
Detector: UV 254 nm
100 % Water Compatible
Good Tolerance of Biological Matrix
A Slightly Polar, 100% Water Compatible and Universal Reverse Phase
An unique and universal Unisol C18 HPLC phase made by Bonna-Agela's patented Unisol Technology. This stationary
phase demonstrated unprecedented separation performance for compounds with a wide range of properties from
hydrophilic to hydrophobic, polar, semi-polar and non-polar compounds. We also offer C8 phase presenting different
selections for various applications.
● Great inertness and separation efficiency for basic compounds
● Enhanced retention of hydrophilic compounds
● 100% aqueous compatibility
● Robust and reproducible performance
● Wide pH range (1.5-9.0)
● Low bleeding and high sensitivity for LC-MS
Notes: * Optimum pH range is 2.0 to 6.0; Use the column under optimum pH could ensure a prolonged lifetime.